The H2AFY Polyclonal Antibody (CAB7045) offered by Assay Genie is a valuable tool for researchers studying the H2AFY protein, which is involved in gene regulation and chromatin structure. This antibody is produced in rabbits and has been validated for use in Western blot applications, providing accurate and reliable results.H2AFY, also known as macroH2A, is a variant of the histone H2A protein that plays a crucial role in DNA packaging and gene expression regulation. Research has shown that H2AFY is involved in various cellular processes, including embryonic development, X chromosome inactivation, and tumor suppression.
By targeting the H2AFY protein, researchers can gain insights into its functions and potential therapeutic applications.This polyclonal antibody specifically binds to the H2AFY protein, allowing for easy detection and analysis in a variety of cell types and biological samples. Its high reactivity with human samples makes it an ideal choice for studies in molecular biology, cancer research, and epigenetics. With the H2AFY Polyclonal Antibody, researchers can further explore the mechanisms underlying gene regulation and chromatin remodeling, paving the way for new discoveries in the field of genetics and genomics.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
9555
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and macroH2A.1 knockout (KO) 293T cells, using [KO Validated] macroH2A.1 Rabbit pAb (CAB7045) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
