The H2AFV Polyclonal Antibody (CAB17813) is a valuable tool for researchers studying the histone variant H2A.V, which plays a crucial role in chromatin remodeling and gene expression regulation. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the H2AFV protein, researchers can easily detect and analyze its expression in different cell types, making it ideal for studies in epigenetics, gene regulation, and cancer research.H2A.V is a histone variant that is incorporated into nucleosomes and contributes to the dynamic regulation of chromatin structure, ultimately influencing gene transcription and cellular processes.
Understanding the functions and mechanisms of H2AFV is essential for unraveling the complexities of epigenetic regulation and its implications in disease development. This antibody provides researchers with a powerful tool to explore the role of H2A.V in various biological contexts and uncover its potential as a therapeutic target for cancer and other diseases.
Product Name:
Histone H2AFV Rabbit Polyclonal Antibody
SKU:
CAB17813
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human Histone H2AFV (NP_036544.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. Several transcript variants encoding different isoforms, have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
94239
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Histone H2AFV Rabbit pAb (CAB17813) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
