The H2AFB1 Antibody (PAC027289) is a crucial tool for researchers studying the H2AFB1 protein, which plays a key role in chromatin remodeling and gene expression regulation. This polyclonal antibody, generated in rabbits, is highly specific to human samples and has been validated for use in various applications, including Western blotting.By targeting the H2AFB1 protein, this antibody allows for the detection and analysis of protein levels in different cell types, making it a valuable resource for researchers investigating epigenetic mechanisms and gene regulation. Its high reactivity and specificity ensure reliable and reproducible results, making it an essential tool in the fields of molecular biology and genetics.
H2AFB1 is known to be involved in modulating chromatin structure and epigenetic modifications, impacting gene transcription and cellular functions. Research on H2AFB1 is crucial for understanding its role in development, cancer, and other diseases, with potential implications for therapeutic interventions targeting epigenetic pathways. This antibody provides researchers with a valuable tool to further explore the functions and mechanisms of H2AFB1, advancing our knowledge of chromatin biology and gene regulation.
Immunohistochemistry of paraffin-embedded human liver tissue using PACO27289 at dilution of 1:100.
Immunofluorescent analysis of MCF-7 cells using PACO27289 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis.
Synonyms:
Histone H2A-Bbd type 1 (H2A Barr body-deficient) (H2A.B) (H2A.Bbd), H2AFB1
UniProt Protein Function:
H2AFB1: Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis. Belongs to the histone H2A family.Protein type: RNA splicingChromosomal Location of Human Ortholog: Xq28Cellular Component: nucleusMolecular Function: DNA bindingBiological Process: chromatin silencing; mRNA processing; nucleosome assembly
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the most centromeric copy which is in intron 22 of the F8 gene. [provided by RefSeq, Oct 2015]
