The H1Foo Monoclonal Antibody (PACO03459) is a specialized tool for researchers studying the H1Foo protein, a key regulator of stem cell pluripotency. This antibody, produced using hybridoma technology, is highly specific for H1Foo and has been validated for use in immunofluorescence and flow cytometry applications. H1Foo is known for its critical role in maintaining the pluripotent state of stem cells, making it an important target for research in regenerative medicine and developmental biology. By targeting H1Foo, researchers can gain insight into the mechanisms underlying stem cell self-renewal and differentiation.
With its high specificity and reliability, the H1Foo Monoclonal Antibody (PACO03459) is a valuable tool for scientists conducting research in stem cell biology and regenerative medicine. Its ability to accurately detect and quantify H1Foo expression in various cell types makes it an essential component of any study aimed at understanding the complex processes governing stem cell fate.
Antibody Name:
H1FOO Antibody
Antibody SKU:
PACO03459
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC, IF
Recommended Dilutions:
IHC:1:100-1:300, IF:1:200-1:1000
Species Reactivity:
Human
Immunogen:
synthesized peptide derived from human Histone H1 around the non-acetylation site of K25.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
H1FOO: May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling. Belongs to the histone H1/H5 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 3q22.1Cellular Component: nucleolus; nucleusMolecular Function: nucleosomal DNA bindingBiological Process: nucleosome positioning
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a replication-independent histone that is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The related mouse gene is expressed only in oocytes. [provided by RefSeq, Oct 2015]
