The GYPB Polyclonal Antibody (CAB7682) is a valuable tool for researchers studying the GYPB protein, which plays a key role in red blood cell antigens. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By binding to the GYPB protein, this antibody enables researchers to detect and analyze GYPB expression in a variety of cell types.The GYPB protein is important for determining blood group antigens, specifically the MNS blood group system.
Understanding the function and expression of GYPB is essential for studies in transfusion medicine, blood compatibility testing, and population genetics. The GYPB Polyclonal Antibody is a valuable tool for researchers investigating the role of GYPB in blood disorders, hemolytic diseases, and other conditions related to red blood cell antigens.
Product Name:
GYPB Rabbit Polyclonal Antibody
SKU:
CAB7682
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-91 of human GYPB (NP_002091.3).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
10kDa
Observed MW:
25kDa
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. GYPB gene consists of 5 exons and has 97% sequence homology with GYPA from the 5' UTR to the coding sequence encoding the first 45 amino acids. In addition to the M or N and S or s antigens, that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta; also, Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2994
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Human serum, using GYPB Rabbit pAb (CAB7682) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
