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Guinea pig Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

SKU:
GPDL00024
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z1Y6
Sensitivity:
7.6pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B1,CED,DPD1,LAP,Camurati-Engelmann Disease,Latency-associated peptide
Reactivity:
Guinea Pig
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Guinea pig Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

The Guinea Pig Transforming Growth Factor Beta-1 (TGFB1) ELISA Kit is specifically designed for the accurate detection of TGFB1 levels in guinea pig serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.TGFB1 is a key protein involved in regulating cell growth, differentiation, and immune response. It plays a critical role in various biological processes such as wound healing, tissue repair, and immune regulation.

Abnormal TGFB1 levels have been linked to various diseases including cancer, fibrosis, and autoimmune disorders, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.Overall, the Guinea Pig TGFB1 ELISA Kit is an essential tool for researchers seeking to accurately quantify TGFB1 levels in guinea pig samples, leading to a better understanding of its role in health and disease.

Product Name:Guinea pig Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit
Product Code:GPDL00024
Size:96 Assays
Target:TGFb1
Synonyms:TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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