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Guinea pig Nerve Growth Factor (NGF) ELISA Kit

SKU:
GPDL00022
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
H0WC72
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NGFB,Beta-NGF,HSAN5,NGF-B,Beta-Nerve Growth Factor
Reactivity:
Guinea Pig
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Guinea pig Nerve Growth Factor (NGF) ELISA Kit

The Guinea Pig Nerve Growth Factor (NGF) ELISA Kit is specifically designed for the precise measurement of NGF levels in guinea pig serum, plasma, and tissue culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reliable results, making it a valuable tool for various research applications.NGF is a key neurotrophic factor that plays a crucial role in the growth, survival, and differentiation of nerve cells. It is essential for the development and maintenance of the nervous system, making it a valuable target for studying neurodegenerative disorders, nerve regeneration, and other related conditions.

By using the Guinea Pig NGF ELISA Kit, researchers can gain valuable insights into the role of NGF in various physiological and pathological processes, ultimately contributing to a better understanding of neurobiology and potential therapeutic interventions.

Product Name:Guinea pig Nerve Growth Factor (NGF) ELISA Kit
Product Code:GPDL00022
Size:96 Assays
Target:NGF
Synonyms:NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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