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Guinea pig Interleukin 10 (IL-10) ELISA Kit

SKU:
GPDL00012
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
H0VDI2
Sensitivity:
5.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CSIF,IL10A,TGIF,Cytokine Synthesis Inhibitory Factor
Reactivity:
Guinea Pig
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Guinea pig Interleukin 10 (IL-10) ELISA Kit

The Guinea Pig Interleukin-10 (IL-10) ELISA Kit from AssayGenie is a valuable tool for accurately measuring levels of IL-10 in guinea pig serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent choice for various research applications. IL-10 is a key cytokine involved in regulating immune response and inflammation. Its dysregulation has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and cancer.

Therefore, the Guinea Pig IL-10 ELISA Kit is essential for studying the role of IL-10 in these diseases and potentially developing new treatment strategies. Trust AssayGenie's Guinea Pig IL-10 ELISA Kit to help advance your research and gain valuable insights into the complex mechanisms of immune regulation and inflammatory processes in guinea pigs.

Product Name:Guinea pig Interleukin 10 (IL-10) ELISA Kit
Product Code:GPDL00012
Size:96 Assays
Target:IL10
Synonyms:CSIF, IL10A, TGIF, Cytokine Synthesis Inhibitory Factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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