The GUF1 Polyclonal Antibody (PACO22361) is a valuable tool for researchers studying GUF1, a protein involved in mitochondrial RNA processing and translation. This antibody, produced in rabbits, shows high reactivity with human samples and is suitable for use in Western blot experiments. By targeting the GUF1 protein, this antibody allows for the detection and analysis of GUF1 expression in various cell types, making it ideal for investigations in molecular biology and mitochondrial research.
GUF1 is a key player in the regulation of mitochondrial gene expression, making it a crucial component in understanding mitochondrial function and dysfunction. Dysregulation of GUF1 has been implicated in various diseases, including neurological disorders and metabolic conditions. The GUF1 Polyclonal Antibody provides researchers with a powerful tool to explore the role of GUF1 in these pathologies and develop potential therapeutic interventions targeting mitochondrial dysfunction.
Antibody Name:
GUF1 Antibody (PACO22361)
Antibody SKU:
PACO22361
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:50-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from internal of human GUF1.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using GUF1 antibody.
Background:
Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_03137
Synonyms:
GTP-binding protein GUF1 homolog;
UniProt Protein Function:
GUF1: Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner. Belongs to the GTP-binding elongation factor family. LepA subfamily.Protein type: Mitochondrial; EC 3.6.5.-Chromosomal Location of Human Ortholog: 4p12Cellular Component: mitochondrial matrix; mitochondrial inner membraneMolecular Function: GTPase activity; GTP binding; ribosome bindingBiological Process: positive regulation of translation; translation
