The GUCY2D Polyclonal Antibody (CAB3605) is a valuable tool for researchers studying GUCY2D, a key protein involved in the regulation of vision and retinal function. This antibody, produced in rabbits, exhibits high reactivity specifically with human samples and has been validated for use in Western blot applications. By targeting the GUCY2D protein, this antibody enables accurate detection and analysis in various cell types, making it an essential component for investigations in ophthalmology and vision-related research.GUCY2D, also known as retinal guanylyl cyclase 1, is essential for regulating the production of cyclic GMP, a critical second messenger in phototransduction processes in the retina.
Mutations in the GUCY2D gene have been associated with various retinal diseases, including Leber congenital amaurosis and retinitis pigmentosa. Understanding the function and regulation of GUCY2D is crucial for developing targeted therapies for these sight-threatening conditions, making the GUCY2D Polyclonal Antibody an indispensable tool for researchers in the field of ophthalmology.
Product Name:
GUCY2D Rabbit Polyclonal Antibody
SKU:
CAB3605
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 52-350 of human GUCY2D (NP_000171.1).
This gene encodes a retina-specific guanylate cyclase, which is a member of the membrane guanylyl cyclase family. Like other membrane guanylyl cyclases, this enzyme has a hydrophobic amino-terminal signal sequence followed by a large extracellular domain, a single membrane spanning domain, a kinase homology domain, and a guanylyl cyclase catalytic domain. In contrast to other membrane guanylyl cyclases, this enzyme is not activated by natriuretic peptides. Mutations in this gene result in Leber congenital amaurosis and cone-rod dystrophy-6 diseases.
Purification Method:
Affinity purification
Gene ID:
3000
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GUCY2D antibody (CAB3605) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
