Guanine-N7_meth Recombinant Protein SARS-Cov-2 (RPES0009)
- SKU:
- RPES0009
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
Background
The nonstructural protein (nsp) 14 of SARS-CoV 2 was identified as a cap (guanine-N7)-methyltransferase (N7-MTase). Nsp14 of coronaviruses two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity. It may be involved in the proof-reading ability during the viral RNA replication and transcription. GTP, dGTP as well as cap analogs GpppG, GpppA and m7GpppG could be methylated by nsp14.positive-stranded RNA genome of the coronaviruses is translated from ORF1 to yield polyproteins that are proteolytically processed into intermediate and mature nonstructural proteins (nsps). SARS-CoV 2 polyproteins incorporate 16 protein domains (nsps). The putative non-structural protein 2 (nsp2) of SARS-CoV plays an important role in viral transcription and replication, and is an attractive target for anti-SARS drug development.
| Information | Description |
| Product Name: | SARS-Cov-2 Guanine-N7_meth Protein (His Tag) |
| Size: | 10µg |
| SKU: | RPES0009 |
| Activity: | |
| Synonym: | SARS-CoV 2 nsp14; SARS-CoV 2 ExoN; Guanine-N7 methyltransferase |
| Protein Construction: | SARS-Cov-2 Guanine-N7 methyltransferase is produced by our E.coli expression system and the target gene encoding Ala1-Gln527 is expressed with a 6His tag at the N-terminus. |
| Sequence: | Ala1-Gln527 |
| Fusion tag: | N-6His |
| Accession: | YP_009725309.1 |
| Species: | Virus |
| Expressed Host: | E.coli |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C. |
| Purity: | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin: | < 1.0 EU per µg as determined by the LAL method. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Mol Mass: | 62.9 kDa |
| AP Mol Mass: | 60 kDa |
| Formulation: | Supplied as a 0.2 uM filtered solution of PBS, 10% Glycerol, pH 7.4. |
| Reconstitution: | Please refer to the printed manual for detailed information. |
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