The GTF3A Polyclonal Antibody (CAB8426) is a valuable tool for research involving GTF3A, a transcription factor involved in the regulation of gene expression. This antibody, raised in rabbits, is highly specific and validated for use in Western blot and immunohistochemistry applications. It binds to the GTF3A protein, allowing for detection and analysis in various cell types and tissues, making it ideal for studies in molecular biology and gene regulation.GTF3A, also known as General Transcription Factor 3A, is crucial for the initiation of transcription and plays a key role in the regulation of gene expression.
Its involvement in the transcription process makes it a target of interest for researchers studying gene regulation, development, and disease mechanisms. Understanding the function of GTF3A is essential for unraveling the complexities of gene expression and developing targeted therapies for related disorders.
Product Name:
GTF3A Rabbit Polyclonal Antibody
SKU:
CAB8426
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF3A (NP_002088.2).
The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein.
Purification Method:
Affinity purification
Gene ID:
2971
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of 293F, using GTF3A Rabbit pAb (CAB8426) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
