The GTF2A1 Polyclonal Antibody (CAB5345) is a valuable tool for researchers studying the GTF2A1 protein, an essential transcription factor involved in the regulation of gene expression. This antibody, produced in rabbits, shows strong reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the GTF2A1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and gene regulation studies.GTF2A1, also known as general transcription factor IIA subunit 1, is a key player in the transcriptional machinery, playing a crucial role in the initiation of RNA polymerase II-mediated transcription.
Understanding the functions and interactions of GTF2A1 is essential for unraveling the complexities of gene regulation and identifying potential therapeutic targets for diseases involving aberrant gene expression. By using the GTF2A1 Polyclonal Antibody, researchers can gain valuable insights into the mechanisms underlying gene transcription and potentially discover novel strategies for treating genetic disorders and cancer.
Product Name:
GTF2A1 Rabbit Polyclonal Antibody
SKU:
CAB5345
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human GTF2A1 (NP_056943.1).
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.
Purification Method:
Affinity purification
Gene ID:
2957
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GTF2A1 antibody (CAB5345) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
