The GSTT1/GSTT4 Antibody (PACO22347) is a highly specific polyclonal antibody designed for research involving the glutathione S-transferase theta 1 (GSTT1) and glutathione S-transferase theta 4 (GSTT4) proteins. These enzymes play a crucial role in detoxification pathways, particularly in the metabolism of xenobiotics and carcinogens. Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. It binds specifically to the GSTT1 and GSTT4 proteins, allowing for efficient detection and analysis in various cell types. This makes it an ideal tool for studies in toxicology, pharmacology, and cancer research.
The GSTT1 and GSTT4 enzymes are known to be involved in the detoxification of various environmental toxins and drugs, as well as in the protection against oxidative stress. Dysfunction or deficiency of these enzymes has been linked to increased susceptibility to certain diseases, including cancer and neurodegenerative disorders. Understanding the role of GSTT1 and GSTT4 in cellular detoxification processes is key to developing targeted therapies and interventions for mitigating the harmful effects of exposure to harmful substances.
Antibody Name:
GSTT1/GSTT4 Antibody (PACO22347)
Antibody SKU:
PACO22347
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human GSTT1/4.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from Jurkat cells, using GSTT1/4 antibody.
Background:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.
GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family.
UniProt Protein Details:
Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; EC 2.5.1.18
The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015]
