The GSTM5 Polyclonal Antibody (CAB15677) is a valuable tool for researchers studying the GSTM5 protein, a member of the glutathione S-transferase (GST) family involved in detoxification processes within the body. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications.The GSTM5 protein plays a crucial role in protecting cells from oxidative stress and harmful toxins by catalyzing the conjugation of glutathione to a wide range of substrates. Dysregulation of GSTM5 has been linked to various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic interventions.
By targeting the GSTM5 protein, researchers can gain insights into how this enzyme contributes to cellular protection mechanisms and potentially identify new strategies for disease prevention and treatment. The GSTM5 Polyclonal Antibody is a valuable resource for studies in molecular biology, biochemistry, and toxicology, providing researchers with a reliable tool for investigating the functions and mechanisms of GSTM5 in health and disease.
Product Name:
GSTM5 Rabbit Polyclonal Antibody
SKU:
CAB15677
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human GSTM5 (NP_000842.2).
Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Diversification of these genes has occurred in regions encoding substrate-binding domains, as well as in tissue expression patterns, to accommodate an increasing number of foreign compounds.
Purification Method:
Affinity purification
Gene ID:
2949
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GSTM5 Rabbit pAb (CAB15677) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
