The GSTK1 Polyclonal Antibody (CAB19963) is a valuable tool for researchers studying GSTK1, a key enzyme involved in glutathione metabolism and detoxification processes in the cell. This antibody, produced in rabbits, demonstrates high specificity and sensitivity towards human samples, making it suitable for various applications such as Western blot analysis.GSTK1 plays a crucial role in maintaining cellular redox homeostasis and protecting cells from oxidative stress by detoxifying electrophilic compounds through glutathione conjugation. Dysregulation of GSTK1 expression has been implicated in various diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target.
By using the GSTK1 Polyclonal Antibody, researchers can effectively detect and quantify GSTK1 protein levels in different cell types and tissues, providing valuable insights into the role of this enzyme in disease pathogenesis. This antibody is a valuable tool for studies investigating the mechanisms underlying glutathione metabolism and the potential therapeutic applications of targeting GSTK1.
Product Name:
[KO Validated] GSTK1 Rabbit Polyclonal Antibody
SKU:
CAB19963
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1).
This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
373156
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and GSTK1 knockout (KO) 293T cells, using [KO Validated] GSTK1 Rabbit pAb (CAB19963) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
