The GSTA3 Polyclonal Antibody (CAB17491) is a valuable tool for research involving GSTA3, a member of the glutathione S-transferase (GST) family of enzymes known for their role in detoxification and drug metabolism. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for a variety of applications such as Western blotting.GSTA3 is involved in the conjugation of reduced glutathione to a wide range of electrophilic compounds, aiding in the removal of potentially harmful substances from cells.
Its expression level and activity have been linked to drug resistance in cancer cells and may serve as a potential biomarker for drug response in personalized medicine.By targeting GSTA3 with this polyclonal antibody, researchers can gain valuable insights into the enzyme's function in various cellular processes, paving the way for new therapeutic strategies in cancer treatment and drug development. Its reliability and versatility make it a valuable asset for studies in pharmacology, toxicology, and cancer research.
Product Name:
GSTA3 Rabbit Polyclonal Antibody
SKU:
CAB17491
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human GSTA3 (NP_000838.3).
Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined.
Purification Method:
Affinity purification
Gene ID:
2940
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GSTA3 Rabbit pAb (CAB17491) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
