GST Activity Assay Kit (Colorimetric) (BA0028)

SKU:: BA0028
UPC:
Product Type:: Assay
Detection Method:: Colorimetric
Instrument:: Microplate Reader
Sample Type:: Biological Samples (Cell Lysates, Tissues)
Research Area:: Metabolism

€609

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Description

Description

GST Activity Assay Kit - Information

Assay Genie's Glutathione S-transferase, GST activity assay kit (colorimetric) is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.

Applications

Glutathione S-transferase activity determination in biological samples

GST Activity Assay Kit - Key Features

Fast and sensitive. Linear detection range (20 uL sample): 2 to 80 U/L for 10 min reaction at 25°C.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

GST Activity Assay Kit - Data Sheet
Kit Includes	Assay Buffer: 25 mL Glutathione: Powder CDNB: 120 uL
Kit Requires	Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate reader.
Method of Detection	OD340nm
Detection Limit	2 U/L
Samples	Biological samples (e.g. cell lysates, tissues, etc.)
Species	All
Protocol Length	10 min
Size	100 tests
Shelf Life	6 months
Storage	Store all components at 4°C upon receiving

More Details

GLUTATHIONE TRANSFERASE (GST; EC 2.5.1.18) is a multifunctionalenzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites.