The GSS Polyclonal Antibody (CAB13495) is specifically designed for research involving GSS, a key enzyme involved in the synthesis of glutathione, an important antioxidant in cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications, including Western blotting.GSS, also known as glutathione synthetase, plays a critical role in maintaining cellular redox balance and protecting cells from oxidative damage. Research into the function and regulation of GSS is essential for understanding its role in oxidative stress, aging, and various diseases, including neurodegenerative disorders and cancers.
By utilizing the GSS Polyclonal Antibody, researchers can investigate the expression levels and localization of GSS in different cell types and tissues, providing valuable insights into the mechanisms underlying antioxidant defense and potential therapeutic strategies for oxidative stress-related conditions. This antibody is a valuable tool for studies in biochemistry, cell biology, and disease research.
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Purification Method:
Affinity purification
Gene ID:
2937
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Glutathione Synthetase (Glutathione Synthetase (GSS)) antibody (CAB13495) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
