The GSS Polyclonal Antibody (CAB1069) is a specialized research tool designed for studying the GSS protein, a key enzyme involved in the metabolism of glutathione. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the GSS protein, researchers can easily detect and analyze its expression in various cell types, making it an ideal tool for investigations in the fields of biochemistry and molecular biology.GSS, also known as glutathione synthetase, plays a crucial role in maintaining cellular redox balance and detoxification processes by producing glutathione, a vital antioxidant.
Dysregulation of GSS activity has been associated with oxidative stress-related diseases, making it a potential therapeutic target for conditions such as neurodegenerative disorders, cardiovascular diseases, and liver dysfunction. By studying the function of GSS using this antibody, researchers can gain insights into the mechanisms underlying these pathological conditions and develop strategies for targeted interventions.
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.
Purification Method:
Affinity purification
Gene ID:
2937
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Glutathione Synthetase (Glutathione Synthetase (GSS)) antibody (CAB1069) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
