The GSK3B Polyclonal Antibody (CAB2081) is a vital tool for researchers studying GSK3B, a key signaling molecule involved in various cellular processes. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the GSK3B protein, this antibody enables researchers to detect and analyze GSK3B expression in different cell types, making it ideal for investigations in cell signaling pathways and disease mechanisms.
GSK3B, also known as glycogen synthase kinase 3 beta, is a crucial regulator of numerous cellular functions, including cell division, metabolism, and apoptosis. Dysregulation of GSK3B has been linked to various diseases, such as cancer, diabetes, and Alzheimer's disease, making it a promising target for therapeutic interventions. By elucidating the role of GSK3B in these conditions, researchers can potentially uncover new treatment strategies and therapeutic targets for improving human health.
Product Name:
GSK3β Rabbit Polyclonal Antibody
SKU:
CAB2081
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-420 of human GSK3β (NP_001139628.1).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
GSK3B; gsk-3β; GSK3β
Positive Sample:
Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, Nucleus.
Calculated MW:
47kDa
Observed MW:
46kDa
The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.
Purification Method:
Affinity purification
Gene ID:
2932
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse brain, using GSK3β Rabbit pAb (CAB2081) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
