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GRK7 Colorimetric Cell-Based ELISA

SKU:
CBCAB00389
Product Type:
ELISA Kit
ELISA Type:
Cell Based
Research Area:
Signal Transduction
Reactivity:
Human
Detection Method:
Colorimetric
€599
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Description

system_update_altDatasheetMSDS

GRK7 Colorimetric Cell-Based ELISA

The GRK7 Colorimetric Cell-Based ELISA Kit is a highly reliable and sensitive assay designed for the detection of G protein-coupled receptor kinase 7 (GRK7) levels in cell lysates. With its high specificity and accuracy, this kit provides researchers with consistent and reproducible results, making it an essential tool for studying the function of GRK7 in various cellular processes.GRK7 is a key regulator of GPCR signaling pathways, influencing processes such as neurotransmission, hormone secretion, and immune response. Aberrant GRK7 activity has been linked to various diseases, including cardiovascular disorders and neurological conditions, making it an important target for drug development and therapeutic intervention.

By accurately measuring GRK7 levels in cell lysates, researchers can gain valuable insights into its role in cellular signaling pathways and disease progression. The GRK7 Colorimetric Cell-Based ELISA Kit offers a straightforward and efficient method for studying GRK7 expression levels, facilitating further research into the molecular mechanisms underlying GPCR signaling and its therapeutic implications.

Product Name:GRK7 Colorimetric Cell-Based ELISA
Product Code:CBCAB00389
ELISA Type:Cell-Based
Target:GRK7
Reactivity:Human
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nmStorage/Stability:4°C/6 Months
Format:96-Well Microplate

The GRK7 Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect GRK7 protein expression profile in cells. The kit can be used for measuring the relative amounts of GRK7 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on GRK7.

Qualitative determination of GRK7 concentration is achieved by an indirect ELISA format. In essence, GRK7 is captured by GRK7-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 131890, UniProt ID: Q8WTQ7, OMIM: 606987, Unigene: Hs.680654
Gene Symbol:GRK7
Sub Type:None
UniProt Protein Function:GRK7: Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
UniProt Protein Details:

Protein type:EC 2.7.11.14; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 3q24

Molecular Function:rhodopsin kinase activity

Biological Process: protein amino acid autophosphorylation; regulation of rhodopsin mediated signaling

NCBI Summary:This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation. [provided by RefSeq, Jul 2008]
UniProt Code:Q8WTQ7
NCBI GenInfo Identifier:21263659
NCBI Gene ID:131890
NCBI Accession:Q8WTQ7.1
UniProt Related Accession:Q8WTQ7
Molecular Weight:62,212 Da
NCBI Full Name:Rhodopsin kinase
NCBI Synonym Full Names:G protein-coupled receptor kinase 7
NCBI Official Symbol:GRK7  
NCBI Official Synonym Symbols:GPRK7  
NCBI Protein Information:rhodopsin kinase
UniProt Protein Name:Rhodopsin kinase
UniProt Synonym Protein Names:G protein-coupled receptor kinase 7; G protein-coupled receptor kinase GRK7
Protein Family:Rhodopsin kinase
UniProt Gene Name:GRK7  
UniProt Entry Name:GRK7_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies (Anti-GRK7 Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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