The GRK7 Polyclonal Antibody (PACO64263) is a valuable tool for researchers studying G protein-coupled receptor kinase 7 (GRK7), a crucial player in the regulation of visual phototransduction. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting GRK7 in human samples, making it ideal for Western blot applications.GRK7 is primarily expressed in the retina, where it is involved in the desensitization of visual pigments in response to light stimulation. Dysregulation of GRK7 activity has been implicated in various vision disorders, making it a promising target for therapeutic interventions aimed at restoring visual function.
By using the GRK7 Polyclonal Antibody, researchers can explore the role of GRK7 in visual signal transduction pathways and gain insight into its potential as a target for the treatment of retinal diseases. Its reliability and accuracy make it a valuable tool for studies focusing on vision impairment and retinal degeneration.
Antibody Name:
GRK7 Antibody (PACO64263)
Antibody SKU:
PACO64263
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
IF:1:100-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Rhodopsin kinase protein (342-550AA)
Immunofluorescence staining of Hela cells with PACO64263 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).
GRK7: Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
UniProt Protein Details:
Protein type:EC 2.7.11.14; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; AGC group; GRK family; GRK subfamily
Chromosomal Location of Human Ortholog: 3q24
Molecular Function:rhodopsin kinase activity
Biological Process: protein amino acid autophosphorylation; regulation of rhodopsin mediated signaling
NCBI Summary:
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation. [provided by RefSeq, Jul 2008]
