The GRK7 Polyclonal Antibody (PAC064115) is a valuable tool for researchers studying G protein-coupled receptor kinase 7 (GRK7), a key player in the regulation of visual phototransduction in the retina. This antibody, generated in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for use in Western blotting and immunofluorescence applications.GRK7 plays a crucial role in the visual signaling pathway by phosphorylating and desensitizing rhodopsin, the light-sensitive receptor in rod cells.
Dysregulation of GRK7 has been associated with vision disorders such as retinitis pigmentosa, highlighting its importance in maintaining proper visual function. By using the GRK7 Polyclonal Antibody, researchers can gain insights into the mechanisms governing visual phototransduction and potentially identify novel therapeutic targets for retinal diseases.
Antibody Name:
GRK7 Antibody (PACO64115)
Antibody SKU:
PACO64115
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Rhodopsin kinase protein (342-550AA)
Immunofluorescence staining of Hela cells with PACO64115 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).
GRK7: Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
UniProt Protein Details:
Protein type:EC 2.7.11.14; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; AGC group; GRK family; GRK subfamily
Chromosomal Location of Human Ortholog: 3q24
Molecular Function:rhodopsin kinase activity
Biological Process: protein amino acid autophosphorylation; regulation of rhodopsin mediated signaling
NCBI Summary:
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation. [provided by RefSeq, Jul 2008]
