The GRK4 Polyclonal Antibody (CAB10370) is a specialized research tool designed for investigating G protein-coupled receptor kinase 4 (GRK4), a vital enzyme involved in regulating signal transduction. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for Western blot applications. By binding to the GRK4 protein, this antibody enables precise detection and analysis in various cell types, facilitating research in areas such as cardiovascular biology and hypertension.
GRK4 is a key player in the regulation of G protein-coupled receptors, which are pivotal in processes like neurotransmission, hormone secretion, and sensory perception. Dysregulation of GRK4 has been linked to various diseases, including cardiovascular disorders and hypertension. By studying the function of GRK4, researchers can gain insights into potential therapeutic targets for treating these conditions and further understanding the mechanisms underlying them.
Product Name:
GRK4 Rabbit Polyclonal Antibody
SKU:
CAB10370
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 359-578 of human GRK4 (NP_892027.2).
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2868
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GRK4 antibody (CAB10370) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
