Anti-GRIN1 Antibody (CAB7167)

Antibody Name:	Anti-GRIN1 Antibody
Antibody SKU:	CAB7167
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 600-700 of human GRIN1 (NP_015566.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	C6, PC-12

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 600-700 of human GRIN1 (NP_015566.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALTL SSAM WFSW GVLL NSGI GEGA PRSF SARI LGMV WAGF AMII VASY TANL AAFL VLDR PEER ITGI NDPR LRNP SDKF IYAT VKQS SVDI YFRR QVEL S
Gene ID:	2902
Uniprot:	Q05586
Cellular Location:	Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, postsynaptic density, synapse
Calculated MW:	99kDa/101kDa/103kDa/105kDa/106kDa/107kDa
Observed MW:	105kDa

Synonyms:

GRIN1, GluN1, MRD8, NMD-R1, NMDA1, NMDAR1, NR1, NMDA 1

Background:

The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	NMDAR1: a subunit of N-methyl-D-aspartate (NMDA) receptors, members of the glutamate receptor channel superfamily. Possesses high calcium permeability and voltage-dependent sensitivity to magnesium and is modulated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. Three alternatively-spliced isoforms have been described.
UniProt Protein Details:	Protein type:Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral; Channel, calcium Chromosomal Location of Human Ortholog: 9q34.3 Cellular Component: neuron projection; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; terminal button; excitatory synapse; N-methyl-D-aspartate selective glutamate receptor complex; postsynaptic membrane; synaptic vesicle; plasma membrane; synapse; cell junction Molecular Function:voltage-gated cation channel activity; neurotransmitter binding; glutamate receptor binding; calcium channel activity; calcium ion binding; calmodulin binding; protein binding; enzyme binding; glutamate binding; extracellular-glutamate-gated ion channel activity; protein heterodimerization activity; N-methyl-D-aspartate selective glutamate receptor activity; glycine binding Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; male mating behavior; prepulse inhibition; adult locomotory behavior; positive regulation of apoptosis; regulation of dendrite morphogenesis; rhythmic process; response to morphine; sensory perception of pain; regulation of axonogenesis; calcium ion homeostasis; synaptic transmission; regulation of respiratory gaseous exchange; conditioned taste aversion; ephrin receptor signaling pathway; visual learning; negative regulation of neuron apoptosis; protein tetramerization; cation transport; synaptic transmission, glutamatergic; response to amphetamine; social behavior; respiratory gaseous exchange; pons maturation; cellular calcium ion homeostasis; regulation of membrane potential; response to ethanol; regulation of synaptogenesis; olfactory learning; long-term memory; suckling behavior; propylene metabolic process; ionotropic glutamate receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; regulation of excitatory postsynaptic membrane potential; response to calcium ion Disease: Mental Retardation, Autosomal Dominant 8
NCBI Summary:	The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	Q05586
NCBI GenInfo Identifier:	548377
NCBI Gene ID:	2902
NCBI Accession:	Q05586.1
UniProt Secondary Accession:	Q05586,P35437, Q12867, Q12868, Q5VSF3, Q5VSF4, Q5VSF5 Q5VSF6, Q5VSF7, A6NLK7, A6NLR1, C9K0X1,
UniProt Related Accession:	Q05586
Molecular Weight:	101,851 Da
NCBI Full Name:	Glutamate receptor ionotropic, NMDA 1
NCBI Synonym Full Names:	glutamate receptor, ionotropic, N-methyl D-aspartate 1
NCBI Official Symbol:	GRIN1
NCBI Official Synonym Symbols:	NR1; MRD8; GluN1; NMDA1; NMDAR1
NCBI Protein Information:	glutamate receptor ionotropic, NMDA 1; NMD-R1; glutamate [NMDA] receptor subunit zeta 1; glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; N-methyl-D-aspartate receptor channel, subunit zeta-1
UniProt Protein Name:	Glutamate receptor ionotropic, NMDA 1
UniProt Synonym Protein Names:	Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NMD-R1
Protein Family:	Glutamate receptor ionotropic
UniProt Gene Name:	GRIN1
UniProt Entry Name:	NMDZ1_HUMAN

	Western blot analysis of extracts of various cell lines, using GRIN1 antibody (CAB7167). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of rat brain using GRIN1 antibody (CAB7167) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of SKOV3 cells using GRIN1 antibody (CAB7167) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-GRIN1 Antibody (CAB13995)	Human Glutamate [NMDA] receptor subunit zeta-1 (GRIN1) ELISA Kit
Anti-GRIN1 Antibody (CAB7677)	NMDAR1 Colorimetric Cell-Based ELISA Kit
Anti-Phospho-GRIN1-S896 Antibody (CABP0165)	NMDAR1 (Phospho-Ser896) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-GRIN1-S890 pAb Antibody (CABP0826)	NMDAR1 (Phospho-Ser897) Colorimetric Cell-Based ELISA Kit
Anti-NMDAR1 Antibody (CAB11699)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)