The GRB2 Polyclonal Antibody (CAB5689) is a valuable tool for researchers studying the GRB2 protein, a key player in signal transduction pathways involved in cell growth and differentiation. This antibody is produced in rabbits and has been shown to have high reactivity with human samples, making it ideal for use in Western blot applications.GRB2 is a crucial adaptor protein that mediates signaling events downstream of receptor tyrosine kinases, leading to activation of various downstream effectors. Understanding the role of GRB2 in these signaling pathways is essential for elucidating cellular processes and identifying potential targets for therapeutic intervention.
With its specificity and sensitivity in detecting GRB2 protein, the CAB5689 Polyclonal Antibody is a valuable tool for researchers in the fields of cell biology, cancer research, and signal transduction studies. Its ability to accurately detect GRB2 protein levels in various cell types makes it an essential reagent for investigating the intricate mechanisms of cell signaling and its dysregulation in disease states.
Product Name:
GRB2 Rabbit Polyclonal Antibody
SKU:
CAB5689
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRB2 (NP_002077.1).
The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2885
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GRB2 antibody (CAB5689) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
