The GPX4 Polyclonal Antibody (CAB1933) is a vital tool for researchers studying the GPX4 protein, an essential enzyme that plays a critical role in protecting cells from oxidative damage. This antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for use in Western blot applications.GPX4, also known as glutathione peroxidase 4, is a key player in the regulation of cell survival and death, making it a promising target for studies in cancer biology, neurodegenerative diseases, and metabolic disorders. By binding specifically to the GPX4 protein, this antibody enables precise detection and analysis in a variety of cell types, providing researchers with valuable insights into the function and regulation of GPX4.
Understanding the role of GPX4 in cellular processes is essential for developing novel therapeutic strategies aimed at manipulating oxidative stress and cell death pathways. With its high specificity and sensitivity, the GPX4 Polyclonal Antibody is a valuable tool for advancing research in the fields of molecular biology, biochemistry, and pharmacology.
Product Name:
GPX4 Rabbit Polyclonal Antibody
SKU:
CAB1933
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 74-197 of GPX4 (NP_002076.2).
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Transcript variants resulting from alternative splicing or use of alternate promoters have been described to encode isoforms with different subcellular localization.
Purification Method:
Affinity purification
Gene ID:
2879
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87 MG cells using GPX4 Rabbit pAb (CAB1933) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
