The GPX3 Polyclonal Antibody (CAB12956) is a valuable tool for research involving GPX3, a member of the glutathione peroxidase family known for its role in antioxidant defense and protecting cells from oxidative damage. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in various applications including Western blotting.GPX3 is a crucial enzyme that helps detoxify harmful reactive oxygen species, making it a key player in maintaining cellular health and protecting against oxidative stress-related diseases. Research into GPX3 can provide insights into conditions such as cancer, cardiovascular disease, and neurodegenerative disorders where oxidative damage plays a significant role.
By utilizing the GPX3 Polyclonal Antibody, researchers can accurately detect and analyze GPX3 expression in different cell types, aiding in the understanding of its function and potential therapeutic applications. Its high reactivity and specificity make it an essential tool for studies in biochemistry, molecular biology, and clinical research aimed at unraveling the complex role of GPX3 in health and disease.
Product Name:
GPX3 Rabbit Polyclonal Antibody
SKU:
CAB12956
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GPX3 (NP_002075.2).
Sequence:
QSRG QEKS KMDC HGGI SG
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
GPx-P; GSHPx-3; GSHPx-P; GPX3
Positive Sample:
Mouse kidney
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
26kDa
Observed MW:
25kDa
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is secreted, and is abundantly found in plasma. Downregulation of expression of this gene by promoter hypermethylation has been observed in a wide spectrum of human malignancies, including thyroid cancer, hepatocellular carcinoma and chronic myeloid leukemia. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2878
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of Mouse kidney, using GPX3 antibody (CAB12956) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
