The GPX1 Rabbit Monoclonal Antibody is a powerful tool for researchers studying GPX1, an essential enzyme that plays a key role in protecting cells from oxidative damage. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting GPX1 in a variety of samples, making it an ideal choice for applications such as Western blotting.GPX1, also known as glutathione peroxidase 1, is a crucial antioxidant enzyme that helps to maintain cellular health by scavenging harmful free radicals. Dysregulation of GPX1 has been implicated in various diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions.
By targeting GPX1, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop novel therapeutic strategies.With its ability to accurately detect GPX1 in biological samples, the GPX1 Rabbit Monoclonal Antibody is a valuable tool for researchers in the fields of biochemistry, molecular biology, and biomedical science. Its reliable performance and proven efficacy make it an indispensable resource for studies aimed at unraveling the complexities of oxidative stress and its impact on human health.
Product Name:
GPX1 Rabbit Monoclonal Antibody
SKU:
CAB11166
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-203 of human GPX1 (NP_000572.2).
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. This gene contains an in-frame GCG trinucleotide repeat in the coding region, and three alleles with 4, 5 or 6 repeats have been found in the human population. The allele with 4 GCG repeats has been significantly associated with breast cancer risk in premenopausal women. Alternatively spliced transcript variants have been found for this gene. Pseudogenes of this locus have been identified on chromosomes X and 21.
Purification Method:
Affinity purification
Gene ID:
2876
Clone Number:
ARC2653
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using GPX1 Rabbit mAb (CAB11166) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
