The GPX1 Polyclonal Antibody (CAB1110) is specifically designed for research involving GPX1, a key enzyme involved in protecting cells from oxidative damage. This antibody, raised in rabbits, exhibits high reactivity with human GPX1 protein and has been validated for use in Western blot applications. By binding to the GPX1 protein, this antibody enables researchers to effectively detect and analyze GPX1 expression in various cell types, making it an invaluable tool for studies in oxidative stress, antioxidant defense mechanisms, and diseases related to oxidative damage.
GPX1, also known as glutathione peroxidase 1, plays a crucial role in cellular defense against reactive oxygen species by catalyzing the reduction of hydrogen peroxide and lipid hydroperoxides. Dysregulation of GPX1 activity has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. The GPX1 Polyclonal Antibody offers researchers a valuable tool for investigating the role of GPX1 in oxidative stress-related diseases and developing potential therapeutic interventions targeting GPX1 activity.
Product Name:
GPX1 Rabbit Polyclonal Antibody
SKU:
CAB1110
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human GPX1 (NP_000572.2).
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. This gene contains an in-frame GCG trinucleotide repeat in the coding region, and three alleles with 4, 5 or 6 repeats have been found in the human population. The allele with 4 GCG repeats has been significantly associated with breast cancer risk in premenopausal women. Alternatively spliced transcript variants have been found for this gene. Pseudogenes of this locus have been identified on chromosomes X and 21.
Purification Method:
Affinity purification
Gene ID:
2876
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GPX1 Rabbit pAb (CAB1110) at 1:1000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Negative control (NC): MCF7.Exposuretime: 60s.
