The GPT Polyclonal Antibody (CAB2814) is a valuable tool for researchers studying the enzyme glutamate pyruvate transaminase (GPT), also known as alanine aminotransferase (ALT). This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it an ideal choice for Western blotting applications.GPT is an essential enzyme involved in amino acid metabolism and plays a key role in the conversion of alanine and α-ketoglutarate to pyruvate and glutamate. Abnormal levels of GPT in the blood are indicative of liver damage and are commonly used as a biomarker for liver function in diagnostic tests.
By utilizing the GPT Polyclonal Antibody, researchers can effectively detect and analyze GPT protein levels in various cell types and tissues. This antibody offers a valuable tool for investigating the role of GPT in liver diseases, metabolic disorders, and other conditions where GPT activity is dysregulated. Studies utilizing this antibody may provide valuable insights for the development of diagnostic tools and therapeutic interventions targeting GPT-related pathways.
Product Name:
GPT Rabbit Polyclonal Antibody
SKU:
CAB2814
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human GPT (NP_005300.1).
This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
Purification Method:
Affinity purification
Gene ID:
2875
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GPT antibody (CAB2814) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
