The GPR50 Polyclonal Antibody (PACO00877) is a powerful tool for research targeting G protein-coupled receptor 50 (GPR50), a key player in the regulation of circadian rhythms and sleep patterns. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it an ideal choice for various research applications, including Western blot analysis.GPR50 is a critical component of the circadian clock, influencing the body's internal clock and sleep-wake cycles. Dysregulation of GPR50 has been linked to sleep disorders, mood disorders, and metabolic disturbances.
Research into the function and regulation of GPR50 is essential for understanding its role in these physiological processes and developing targeted therapies for related conditions.By using the GPR50 Polyclonal Antibody, researchers can detect and analyze GPR50 expression in a variety of cell types, shedding light on its biological functions and potential as a therapeutic target. This antibody is a valuable tool for studies in neuroscience, chronobiology, and sleep medicine, providing insights into the intricate mechanisms governing circadian rhythms and sleep behavior.
Antibody Name:
GPR50 Antibody
Antibody SKU:
PACO00877
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IF
Recommended Dilutions:
WB:1:500-1:2000, IF:1:200-1:1000
Species Reactivity:
Human
Immunogen:
synthesized peptide derived from the Internal region of human GPR50.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
GPR50; Melatonin-related receptor; G protein-coupled receptor 50; H9
UniProt Protein Function:
GPR50: an orphan G protein coupled receptor with high sequence homology to the melatonin receptors MT1 and MT2. Does not bind melatonin. Forms heterodimers with MT1 and MT2 and acts as a negative regulator of MT1 agonist binding and G protein coupling; inhibition of melatonin receptor activity is dependent on the long, proline-rich carboxy terminal tail of GPR50. On a physiological level, GPR50 is involved in the regulation of adaptive thermoregulation in mammals and deletion of GPR50 in mice produces a profound effect on the response to fasting and facilitates entry into torpor. Polymorphisms in the corresponding GPR50 gene are associated with bipolar affective disorder and major depressive disorder in women, indicating that variation in GPR50 may be an important gender-specific risk factor for certain mental disorders. Belongs to the G-protein coupled receptor 1 family.Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-passChromosomal Location of Human Ortholog: Xq28Cellular Component: integral to plasma membraneMolecular Function: G-protein coupled receptor activity; identical protein binding; protein bindingBiological Process: cell surface receptor linked signal transduction; cell-cell signaling
UniProt Protein Details:
NCBI Summary:
This gene product belongs to the G-protein coupled receptor 1 family. Even though this protein shares similarity with the melatonin receptors, it does not bind melatonin, however, it inhibits melatonin receptor 1A function through heterodimerization. Polymorphic variants of this gene have been associated with bipolar affective disorder in women. [provided by RefSeq, Jan 2010]
