The GPR27 Polyclonal Antibody (PAC061229) is a valuable tool for researchers studying GPR27, a G-protein coupled receptor involved in various physiological processes. This antibody, produced in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for Western blot applications. By targeting GPR27, researchers can investigate its role in signaling pathways and potential implications in disease development.GPR27 is known for its involvement in neurotransmission, cellular signaling, and metabolic regulation. Studies have shown its potential impact on conditions such as neurological disorders, metabolic diseases, and even cancer progression.
By utilizing this antibody, researchers can gain valuable insights into the functions of GPR27 and its potential as a therapeutic target in various disease settings.Overall, the GPR27 Polyclonal Antibody offers researchers a reliable tool for exploring the functions and implications of GPR27 in different biological contexts. Its specificity and sensitivity make it a valuable asset in drug development, disease research, and understanding the intricate mechanisms underlying GPR27-mediated pathways.
IHC image of PACO61229 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO61229 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Orphan receptor. Possible candidate for amine-like G-protein coupled receptor.
GPR27: Orphan receptor. Possible candidate for amine-like G- protein coupled receptor. Belongs to the G-protein coupled receptor 1 family.Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-passChromosomal Location of Human Ortholog: 3p21-p14Cellular Component: plasma membraneBiological Process: signal transduction
UniProt Protein Details:
NCBI Summary:
GPR27 is a member of the G protein-coupled receptors (GPCRs), a large family of receptors that have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters, hormones, or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins.[supplied by OMIM, May 2010]
