The GPR174 Polyclonal Antibody (PACO59509) is a valuable tool for researchers studying GPR174, a G-protein coupled receptor that plays a role in immune regulation and inflammatory responses. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the GPR174 protein, this antibody allows for the detection and analysis of GPR174 in various cell types, making it useful for studies in immunology and cancer research.
GPR174 is known for its involvement in immune function and its potential as a therapeutic target for various diseases, including cancer and inflammatory conditions. Understanding the role of GPR174 in immune regulation is essential for the development of novel therapies that modulate immune responses in these contexts. Researchers can utilize this antibody to explore the function of GPR174 and its implications in disease pathogenesis, ultimately paving the way for new treatment strategies.
Western Blot. Positive WB detected in: HL60 whole cell lysate. All lanes: GPR174 antibody at 2.8µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 kDa. Observed band size: 39 kDa.
Immunofluorescence staining of Hela cells with PACO59509 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO59509 diluted at 1:300 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Putative receptor for purines coupled to G-proteins.
Synonyms:
Probable G-protein coupled receptor 174, GPR174
UniProt Protein Function:
GPR174: Putative receptor for purines coupled to G-proteins. Belongs to the G-protein coupled receptor 1 family.Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCRChromosomal Location of Human Ortholog: Xq21.1
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein belonging to the G protein-coupled receptor superfamily. These proteins are characterized by the presence of seven alpha-helical transmembrane domains, and they activate or interact with various endogenous or exogenous ligands, including neurotransmitters, hormones, and odorant and taste substances. This family member is classified as an orphan receptor because the cognate ligand has not been identified. [provided by RefSeq, Sep 2011]
