The GPR158 Polyclonal Antibody (PAC05602) is a valuable tool for researchers studying GPR158, a G protein-coupled receptor involved in various physiological processes. This antibody is raised in rabbits and has high reactivity with human samples, making it suitable for use in Western blot applications. By binding to the GPR158 protein, this antibody enables the detection and analysis of GPR158 expression in different cell types, making it an ideal choice for studies in neuroscience and pharmacology.GPR158 is known for its role in regulating synaptic plasticity and neuronal signaling, making it a potential target for drug development in neurological disorders such as depression and schizophrenia.
Understanding the function of GPR158 is crucial for unraveling its molecular mechanisms and potential therapeutic applications. Researchers can use this antibody to investigate the role of GPR158 in various cellular processes and disease pathways, advancing our understanding of its significance in health and disease.
IHC image of PACO56402 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with PACO56402 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO56402 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
GPR158: a member of the metabotropic glutamate family of G-protein coupled receptor 3 family.Protein type: GPCR, family 3; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integralChromosomal Location of Human Ortholog: 10p12.1Cellular Component: integral to membrane; plasma membraneMolecular Function: G-protein coupled receptor activityBiological Process: G-protein coupled receptor protein signaling pathway; regulation of G-protein coupled receptor protein signaling pathway
