The GPR143 Polyclonal Antibody (CAB10568) is a valuable tool for researchers studying GPR143, a G protein-coupled receptor known for its role in pigmentation and vision. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the GPR143 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an essential component for studies in ophthalmology, dermatology, and neuroscience.GPR143, also known as the OA1 receptor, is involved in regulating melanin production and distribution in melanocytes, as well as playing a role in eye development and function.
Mutations in the GPR143 gene are associated with ocular albinism, a genetic disorder affecting pigmentation and visual acuity. Research into GPR143 function is key to understanding these processes and developing targeted therapies for pigmentary disorders and vision-related conditions.Overall, the GPR143 Polyclonal Antibody offers researchers a reliable tool for investigating the biology of GPR143 and its implications in pigmentation, vision, and related diseases. Its high specificity and sensitivity make it an indispensable resource for advancing our knowledge in these fields.
Product Name:
GPR143 Rabbit Polyclonal Antibody
SKU:
CAB10568
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 314-404 of human GPR143 (NP_000264.2).
This gene encodes a protein that binds to heterotrimeric G proteins and is targeted to melanosomes in pigment cells. This protein is thought to be involved in intracellular signal transduction mechanisms. Mutations in this gene cause ocular albinism type 1, also referred to as Nettleship-Falls type ocular albinism, a severe visual disorder. A related pseudogene has been identified on chromosome Y.
Purification Method:
Affinity purification
Gene ID:
4935
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GPR143 antibody (CAB10568) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
