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[KD Validated] GPLD1 Polyclonal Antibody (CAB21781)

SKU:
CAB21781
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €529
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KD Validated] GPLD1 Polyclonal Antibody
SKU:CAB21781
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2).
Sequence:CGLS THVE IGHR ALEF LQLH NGRV NYRE LLLE HQDA YQAG IVFP DCFY PSIC KGGK FHDV SEST HWTP FLNA SVHY IREN YPLP WEKD TEKL VAFL FGIT SHMA ADVS WHSL GLEQ GFLR TMGA IDFH GSYS EAHS A
Tested Applications:WB ELISA
Recommended Dilution:WB,1:100 - 1:500
Synonyms:PLD; GPIPLD; PIGPLD; GPIPLDM; PIGPLD1; [KD Validated] GPLD1
Positive Sample:293T,Daudi
Conjugate:Unconjugated
Cellular Localization:Secreted.
Calculated MW:92kDa
Observed MW:92kDa

Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.

Purification Method:Affinity purification
Gene ID:2822
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from Daudi cells, using [KD Validated] GPLD1 Rabbit pAb (CAB21781) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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