The GPER1 Polyclonal Antibody (CAB10217) is a valuable tool for researchers studying the G protein-coupled estrogen receptor 1 (GPER1), a cell surface receptor involved in mediating estrogen signaling pathways. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications.GPER1 plays a crucial role in various physiological processes, including cell proliferation, migration, and survival, as well as hormone-dependent cancers. By targeting the GPER1 protein, this antibody allows for the detection and analysis of GPER1 expression in different cell types, making it an ideal choice for studies in cancer biology, estrogen signaling, and hormone-related diseases.
Understanding the function and regulation of GPER1 is essential for developing targeted therapies for hormone-related disorders, as well as identifying potential biomarkers for disease diagnosis and prognosis. The GPER1 Polyclonal Antibody provides researchers with a reliable tool to advance their understanding of GPER1 biology and its implications in health and disease.
Product Name:
GPER1 Rabbit Polyclonal Antibody
SKU:
CAB10217
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 307-375 of human GPER1 (NP_001496.1).
This gene encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum and a member of the G-protein coupled receptor 1 family. This receptor binds estrogen and activates multiple downstream signaling pathways, leading to stimulation of adenylate cyclase and an increase in cyclic AMP levels, while also promoting intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. This receptor has been shown to play a role in diverse biological processes, including bone and nervous system development, metabolism, cognition, male fertility and uterine function.
Purification Method:
Affinity purification
Gene ID:
2852
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse heart , using GPER1 Rabbit pAb (CAB10217) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
