The GPD1 Polyclonal Antibody (CAB5715) is a research tool specifically designed for studying the enzyme GPD1, which plays a crucial role in glycerol metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the GPD1 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in biochemistry, metabolism, and related fields.GPD1, also known as glycerol-3-phosphate dehydrogenase 1, is a key enzyme involved in the glycerol phosphate shuttle pathway, which plays a critical role in lipid metabolism and energy production.
Dysfunction of GPD1 has been linked to metabolic disorders, including obesity, diabetes, and cardiovascular diseases. Research into the activity and regulation of GPD1 is essential for understanding its role in metabolic pathways and developing potential therapeutic strategies for metabolic disorders.Overall, the GPD1 Polyclonal Antibody (CAB5715) offers researchers a reliable tool for investigating the function and regulation of GPD1 in various physiological and pathological conditions, ultimately contributing to the advancement of knowledge in the field of metabolism and potential implications for disease prevention and treatment.
Product Name:
GPD1 Rabbit Polyclonal Antibody
SKU:
CAB5715
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human GPD1 (NP_005267.2).
This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
2819
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GPD1 antibody (CAB5715) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
