The GP1BB Polyclonal Antibody (CAB10113) is a research tool designed for studying GP1BB, a cell surface glycoprotein involved in platelet activation and adhesion. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting GP1BB in human samples, making it a valuable tool for research in hemostasis and thrombosis.GP1BB, also known as Glycoprotein Ib platelet subunit beta, plays a crucial role in platelet function by mediating the initial interaction between platelets and the subendothelial matrix at sites of vascular injury. Dysregulation of GP1BB has been implicated in various platelet disorders and thrombotic events, highlighting its importance in maintaining vascular integrity and hemostasis.
The GP1BB Polyclonal Antibody is validated for use in techniques such as Western blotting, immunohistochemistry, and flow cytometry, enabling researchers to study GP1BB expression and function in different experimental settings. By understanding the role of GP1BB in platelet biology, researchers can gain insights into thrombotic diseases and develop targeted therapies to manage and treat these conditions effectively.
Product Name:
GP1BB Rabbit Polyclonal Antibody
SKU:
CAB10113
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-147 of human GP1BB (NP_000398.1).
Platelet glycoprotein Ib (GPIb) is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. It is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (VWF), and mediates platelet adhesion in the arterial circulation. GPIb alpha chain provides the VWF binding site, and GPIb beta contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. Mutations in the GPIb beta subunit have been associated with Bernard-Soulier syndrome, velocardiofacial syndrome and giant platelet disorder. The 206 amino acid precursor of GPIb beta is synthesized from a 1.0 kb mRNA expressed in plateletes and megakaryocytes. A 411 amino acid protein arising from a longer, unspliced transcript in endothelial cells has been described; however, the authenticity of this product has been questioned. Yet another less abundant GPIb beta mRNA species of 3.5 kb, expressed in nonhematopoietic tissues such as endothelium, brain and heart, was shown to result from inefficient usage of a non-consensus polyA signal in the neighboring upstream gene (SEPT5, septin 5). In the absence of polyadenylation from its own imperfect site, the SEPT5 gene produces read-through transcripts that use the consensus polyA signal of this gene.
Purification Method:
Affinity purification
Gene ID:
2812
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GP1BB antibody (CAB10113) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
