The GOT1 Rabbit Monoclonal Antibody is a powerful tool for researchers studying the protein GOT1, an enzyme involved in the metabolism of amino acids. This antibody, produced using rabbit monoclonal technology, offers high specificity and sensitivity for detecting GOT1 in various samples, particularly human tissues.GOT1 plays a crucial role in the conversion of aspartate to oxaloacetate, a key step in the urea cycle and amino acid metabolism. Dysregulation of GOT1 has been implicated in various diseases, including cancer and metabolic disorders, making it a promising target for therapeutic interventions.
Validated for use in Western blotting, immunofluorescence, and immunohistochemistry, this antibody enables precise and reliable detection of GOT1 in experimental settings. Its versatility and efficacy make it an essential tool for investigating the role of GOT1 in cellular processes and disease pathogenesis.
Product Name:
GOT1 Rabbit Monoclonal Antibody
SKU:
CAB11363
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human GOT1 (NP_002070.1).
Hep G2,A549,U-87MG,Mouse liver,Mouse brain,Mouse heart,Rat liver,Rat brain,Rat kidney
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm
Calculated MW:
46kDa
Observed MW:
41kDa
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Purification Method:
Affinity purification
Gene ID:
2805
Clone Number:
ARC0579
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using GOT1 Rabbit mAb (CAB11363) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
