The GOT1 Polyclonal Antibody (CAB5822) is a valuable tool for researchers studying the role of the GOT1 protein in cellular metabolism and cancer. GOT1, also known as glutamic-oxaloacetic transaminase 1, is an enzyme involved in amino acid metabolism and the production of energy in cells. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.By binding to the GOT1 protein, this antibody allows for the detection and analysis of GOT1 expression in various cell types, making it ideal for studies in biochemistry and cancer research.
Understanding the function of GOT1 is important for unravelling its role in cancer progression and metabolic reprogramming, which could lead to the development of targeted therapies for cancer treatment.Overall, the GOT1 Polyclonal Antibody is a reliable tool for researchers looking to investigate the biological functions of the GOT1 protein and its implications in disease, ultimately contributing to advancements in the field of cancer research and therapy development.
Product Name:
GOT1 Rabbit Polyclonal Antibody
SKU:
CAB5822
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1).
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Purification Method:
Affinity purification
Gene ID:
2805
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GOT1 Rabbit pAb (CAB5822) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
