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GRASP65 Rabbit Polyclonal Antibody (CAB6609)

SKU:
CAB6609
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • Cell Biology Antibodies 10 Anti-GORASP1 Antibody CAB6609
  • Cell Biology Antibodies 10 Anti-GORASP1 Antibody CAB6609
  • Cell Biology Antibodies 10 Anti-GORASP1 Antibody CAB6609
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:GRASP65 Rabbit Polyclonal Antibody
SKU:CAB6609
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 221-440 of human GRASP65 (NP_114105.1).
Sequence:ALPL GAPP PDAL PPGP TPED SPSL ETGS RQSD YMEA LLQA PGSS MEDP LPGP GSPS HSAP DPDG LPHF METP LQPP PPVQ RVMD PGFL DVSG ISLL DNSN ASVW PSLP SSTE LTTT AVST SGPE DICS SSSS HERG GEAT WSGS EFEV SFLD SPGA QAQA DHLP QLTL PDSL TSAA SPED GLSA ELLE AQAE EEPA STEG LDTG TEAE GLDS QAQI STTE
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:10 - 1:100
Synonyms:P65; GOLPH5; GRASP65
Positive Sample:BT474,U-937
Conjugate:Unconjugated
Cellular Localization:Cytoplasmic side, Golgi apparatus, Peripheral membrane protein, cis-Golgi network membrane.
Calculated MW:46kDa
Observed MW:65kDa

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene.

Purification Method:Affinity purification
Gene ID:64689
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using GRASP65 antibody (CAB6609) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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