The Golga5 Polyclonal Antibody (CAB15768) is specifically designed for research involving Golgi apparatus protein 5 (GOLGA5), a protein that plays a crucial role in regulating the structure and function of the Golgi apparatus within cells. The antibody, generated in rabbits, is highly reactive with human samples and has been validated for use in various applications, including Western blotting.GOLGA5 is involved in the maintenance of Golgi morphology and protein transport, making it a key player in cellular processes such as protein sorting and secretion.
Dysregulation of GOLGA5 has been implicated in diseases such as cancer and neurodegenerative disorders, highlighting its importance in cellular homeostasis.By using the Golga5 Polyclonal Antibody, researchers can easily detect and analyze GOLGA5 protein levels in different cell types, providing valuable insights into its function and potential implications in disease development. This antibody is an essential tool for studies in cell biology, cancer research, and neurobiology, offering a deeper understanding of the role of GOLGA5 in cellular physiology.
Product Name:
GOLGA5 Rabbit Polyclonal Antibody
SKU:
CAB15768
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA5 (NP_005104.3).
Golgi apparatus membrane, Single-pass type IV membrane protein.
Calculated MW:
83kDa
Observed MW:
100kDa,115kDa
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5.
Purification Method:
Affinity purification
Gene ID:
9950
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GOLGA5 Rabbit pAb (CAB15768) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
