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Goat Interleukin 2 (IL-2) ELISA Kit

SKU:
GTDL00010
Product Type:
ELISA Kit
Sensitivity:
6.9pg/mL
Range:
15.6-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TCGF,Lymphokine,T-Cell Growth Factor,Aldesleukin
Reactivity:
Goat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Goat Interleukin 2 (IL-2) ELISA Kit

The Goat Interleukin-2 (IL-2) ELISA Kit is a reliable and accurate tool for detecting levels of interleukin-2 in goat serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it ideal for a variety of research applications.Interleukin-2 is a key cytokine involved in immune response regulation, playing a crucial role in the activation and proliferation of T cells.

Dysregulation of IL-2 levels has been linked to various autoimmune diseases, inflammatory disorders, and cancer, highlighting its importance as a biomarker for studying these conditions and potential therapeutic interventions.Trust the Goat Interleukin-2 (IL-2) ELISA Kit for accurate and reliable measurements of IL-2 levels in goat samples, providing valuable insights for your research projects and experimental studies.

Product Name:Goat Interleukin 2 (IL-2) ELISA Kit
Product Code:GTDL00010
Size:96 Assays
Target:IL2
Synonyms:TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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