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Goat Interleukin 18 (IL-18) ELISA Kit

SKU:
GTDL00008
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3ZT29
Sensitivity:
6.3pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IGIF,IL-1g,IL1F4,IL1-F4,Interferon-Gamma-Inducing Factor,Interleukin-1 Family Member 4,Iboctadekin,Interleukin-1 Gamma
Reactivity:
Goat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Goat Interleukin 18 (IL-18) ELISA Kit

The Goat Interleukin-18 (IL18) ELISA Kit is a highly reliable and efficient tool for detecting IL18 levels in goat serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an ideal choice for various research applications.Interleukin-18 is a key cytokine involved in immune response regulation, inflammation, and other physiological processes.

It plays a crucial role in various inflammatory diseases, autoimmune disorders, and infections, making it a valuable biomarker for studying these conditions and developing targeted therapies.Overall, the Goat Interleukin-18 (IL18) ELISA Kit offers researchers a powerful tool to assess IL18 levels in goat samples accurately, contributing to the advancement of research in immunology, inflammation, and related fields.

Product Name:Goat Interleukin 18 (IL-18) ELISA Kit
Product Code:GTDL00008
Size:96 Assays
Target:IL18
Synonyms:IGIF, IL-1g, IL1F4, IL1-F4, Interferon-Gamma-Inducing Factor, Interleukin-1 Family Member 4, Iboctadekin, Interleukin-1 Gamma
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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