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Goat Interleukin 12B (IL-12B) ELISA Kit

SKU:
GTDL00007
Product Type:
ELISA Kit
Sensitivity:
13.6pg/mL
Range:
31.2-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
p40,IL12-B,CLMF2,NKSF2,Natural Killer Cell Stimulatory Factor 2,Cytotoxic Lymphocyte Maturation Factor 2,Interleukin 23, P40 Subunit
Reactivity:
Goat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Goat Interleukin 12B (IL-12B) ELISA Kit

The Goat Interleukin-12B (IL-12B) ELISA Kit is a reliable tool for the quantitative detection of IL-12B levels in goat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IL-12B, also known as interleukin-12 subunit beta, is a key cytokine involved in regulating immune responses and promoting the differentiation of T cells.

Dysregulation of IL-12B has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its easy-to-use protocol and quick assay time, the Goat IL-12B ELISA Kit is an essential tool for researchers looking to explore the role of IL-12B in immune system function and disease pathology in goats.

Product Name:Goat Interleukin 12B (IL-12B) ELISA Kit
Product Code:GTDL00007
Size:96 Assays
Target:IL12B
Synonyms:p40, IL12-B, CLMF2, NKSF2, Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, Interleukin 23, P40 Subunit
Detection Method:Sandwich
Range:31.2-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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