null

Goat Growth Hormone Releasing Hormone (GHRH) ELISA Kit

SKU:
GTDL00004
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P63293
Sensitivity:
6.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
GRF,GHRF,Somatocrinin,Growth-Hormone-Releasing Factor,Sermorelin,Somatoliberin
Reactivity:
Goat
Sample Type:
Serum, Plasma And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Goat Growth Hormone Releasing Hormone (GHRH) ELISA Kit

The Goat Growth Hormone-Releasing Hormone (GHRH) ELISA Kit is a powerful tool for accurate detection and quantification of GHRH levels in goat serum, plasma, and other biological samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.GHRH is a key regulator of growth hormone release in goats, playing a crucial role in growth and development. Monitoring GHRH levels can provide valuable insights into the endocrine system and help researchers better understand the mechanisms underlying growth disorders and other physiological processes in goats.

With its user-friendly protocol and rapid assay turnaround time, the Goat GHRH ELISA Kit is an essential resource for scientists studying goat physiology, endocrinology, and growth hormone regulation. Get accurate and dependable results with this advanced ELISA kit from AssayGenie.

Product Name:Goat Growth Hormone Releasing Hormone (GHRH) ELISA Kit
Product Code:GTDL00004
Size:96 Assays
Target:GHRH
Synonyms:GRF, GHRF, Somatocrinin, Growth-Hormone-Releasing Factor, Sermorelin, Somatoliberin
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products