The GNPTAB Polyclonal Antibody (CAB15895) is a valuable tool for researchers studying GNPTAB, a critical gene involved in the biosynthesis of lysosomal enzymes. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the GNPTAB protein, this antibody enables the detection and analysis of GNPTAB in various cell types, making it an excellent choice for studies in biochemistry and molecular biology.GNPTAB plays a crucial role in lysosomal enzyme targeting and sorting, making it essential for proper lysosomal function.
Mutations in the GNPTAB gene are associated with lysosomal storage disorders, highlighting its significance in cellular processes. Research on GNPTAB is vital for understanding lysosomal biology and developing therapies for lysosomal storage disorders.Overall, the GNPTAB Polyclonal Antibody (CAB15895) is a valuable tool for uncovering the role of GNPTAB in cellular function and disease pathology. Its high specificity and reactivity make it a reliable choice for researchers working in the fields of biochemistry, molecular biology, and lysosomal storage disorders.
Product Name:
GNPTAB Rabbit Polyclonal Antibody
SKU:
CAB15895
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-350 of human GNPTAB (NP_077288.2).
Golgi apparatus membrane, Single-pass type I membrane protein, Single-pass type II membrane protein.
Calculated MW:
144kDa
Observed MW:
144kDa
This gene encodes two of three subunit types of the membrane-bound enzyme N-acetylglucosamine-1-phosphotransferase, a heterohexameric complex composed of two alpha, two beta, and two gamma subunits. The encoded protein is proteolytically cleaved at the Lys928-Asp929 bond to yield mature alpha and beta polypeptides while the gamma subunits are the product of a distinct gene (GeneID 84572). In the Golgi apparatus, the heterohexameric complex catalyzes the first step in the synthesis of mannose 6-phosphate recognition markers on certain oligosaccharides of newly synthesized lysosomal enzymes. These recognition markers are essential for appropriate trafficking of lysosomal enzymes. Mutations in this gene have been associated with both mucolipidosis II and mucolipidosis IIIA.
Purification Method:
Affinity purification
Gene ID:
79158
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GNPTAB Rabbit pAb (CAB15895) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
